Food Chemistry (CHIM/10)

Attività di ricerca

Foods, necessary to provide energy and maintain a good state of health, are a source of functional molecules important for the prevention of diseases onset and progression of degenerative states that can result in real diseases. The research of this group is aimed to the study of foods with high nutritional value, such as blueberries, pomegranates, kiwifruits, olive and seed oils, goji berries, strawberries, saffron and many others, using traditional and advanced methodologies. In this way, it is possible to highlight the most interesting compounds responsible for foods healthy properties.
The research is carried out through the study of the food metabolic profile using untargeted methodologies such as Nuclear Resonance spectroscopy (NMR), which allows to obtain the overall profile of the metabolites (metabolomics) present in the samples and targeted methodologies (HPLC-DAD, GC, colorimetry, UV-vis spectrophotometry, etc.), used specifically for selected classes of compounds.

The proposed analytical protocol, together with suitable chemometric methods, allowed the characterization of different foodstuffs in terms of geographical origin, variety, genuineness and production technology and highlighted the presence of molecules of high biological interest that can be tested to evaluate the possible properties for disease prevention.

Collaborazioni nazionali e internazionali
  • Prof. Andrei Mocan, (Department of Pharmaceutical Botany, Iuliu Hațieganu University of Medicine and Pharmacy Cluj-Napoca, Romania)
  • Dr. Simone Carradori and Dr. Marcello Locatelli (Dipartimento di Farmacia dell'Università ‘‘G. d’Annunzio” di Chieti-Pescara)
  • Prof. Benayache (Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine, Algeria)
  • Prof. Maria Daglia (Università di Pavia, Dipartimento di Scienze del Farmaco)
  • Dr. Noemi Proietti, Dr.Anatoly P. Sobolev, Dr. Valeria di Tullio, Istituto per i Sistemi Biologici, CNR, Rome
  • Prof, Giacomo Dugo, Università di Messina, Dipartimento di Scienze biomediche, odontoiatriche e delle immagini morfologiche e funzionali
  • Dr. Nicola Cicero, Università di Messina, Dipartimento di Scienze biomediche, odontoiatriche e delle immagini morfologiche e funzionali
  • Dr. Archimede Rotondo Università di Messina, Dipartimento di Scienze biomediche, odontoiatriche e delle immagini morfologiche e funzionali
  • Perkin Elmer HPLC with DAD
  • UV and Fl detectors
  • Perkin Elmer spectrophotometry UV-vis and X-Rite colorimeter
  • Automated Microwave Biotage Initiator equipped with IR sensor for temperature control (Laboratory 108).
  • 600 MHz Jeol spectrometer  for NMR in solution 
  • 400 MHz Bruker Spectrometer for NMR in solution
Settori ERC
  • LS2_4 - Metabolomics
  • LS9_6 - Food sciences
  • PE4_2 - Spectroscopic and spectrometric techniques
  • 2019 University Research (RM11916B7A7ED51B). Title: Identificazione di biomarcatori di aderenza alla Dieta Mediterranea in una popolazione universitaria di Sapienza (GONG) mediante analisi metabolomica NMR.
  • 2019 Univesity Research (RP11916B73FBB39F)Title: Valutazione degli effetti dei trattamenti tecnologici sulla componente polifenolica delle fragole
  • 2018. Contract between Dep, CTF and Basso Fedele e Figli Srl. Grant (€) 50000 + IVA
  • 2018 University Research  (RP1181643691A1452018)Title: Valutazione della qualità di oli extra vergine di oliva presenti sul mercato italiano
  • 2018-2020 L.R. 13/2008 - Progetti di Gruppi di Ricerca". CUP:    B86C1800073002, 85201715069. Title: La Canapa industriale: sviluppo e valorizzazione di una nuova filiera agroalimentare ecosostenibile: Grant  150000 Euro, Ente Finanziatore: Rezione Lazio
  • 2018 Univesrsity project  (Ateneo, anno 2018, n. prot RG1181642723114C.)Titolo: A multimethodological pilot study to develop personalized diets including functional plant food/beverages and appropriate physical training, for young obese adults. Importo: 35000 Euro
  • 2017. University Research ( RM11715C77E550EB) Title: Studio multi-metodologico dei prodotti della filiera agroalimentare della canapa industriale
  • 2016. University Research. ( RG116154C8E02882) Title: Multi-methodological approach for the characterization of exotic plant foods with high nutritional value
  • 2016. University Research. Bacche di goji (Lycium barbarumL.): un nuovo alimento funzionale.
  • 2015. University Research (Grant: C26A15CJ98) Nuclear Magnetic Resonance-Based Metabolomics Approach in sarcopenic obese subjects: a focus on insulin resistance, inflammation, protein metabolism and muscle strength
  • 2015. University Research (Grant: C26A1524SW) Valutazione dell'attività antiossidante ed antiproliferativa di ellagitannini estratti da Punica granatum L.
  • 2015-2018. Research project ( FILAS-RU-2014-1157, CUP B82I15003570002).  Title: “e-ALIERB: un OPEN LAB per caratterizzare e valorizzare i prodotti alimentari ed erboristici del territorio laziale”.
  • 2013-2018. Seventh Frame work Program THEME [KBBE.2013.2.4-01, Assuring quality and authenticity in the food chain], Grant agreement for: Collaborative project, Project acronym: FOODINTEGRITY, Grant agreement no: 613688. Project full title: " Ensuring the Integrity of the European food chain ".

















Pubblicazioni 2015-2020

1. Cesa, S., Casadei, M.A., Cerreto, F., Paolicelli, P.: “Infant Milk Formulas: effect of storage conditions on the stability of powdered products towards autoxidation”, Foods, 2015, 4, 487-500.

2. Masci, A., Coccia, A., Lendaro, E., Mosca, L., Paolicelli, P., Cesa, S.: “Evaluation of different extraction methods from pomegranate whole fruit and peels and of the antioxidant and antiproliferative activity of the polyphenolic fraction” Food Chem., 2016, 202, 59-69.

3.Mannina, L., Marini, F., Antiochia, R., Cesa, S., Magrì, A., Capitani, D, Sobolev A.P.: “Tracing the origin of beer samples by NMR and chemometrics: Trappist beers as a case of study” Electrophoresis, 2016, 37, 2710-2719.

4.Krasodomska, O., Paolicelli, P., Cesa, S:, Casadei M.A., Jungnickel, C.: “Protection and viability of fruit seeds oils by nanostructured lipid carrier (NLC) nanosuspensions” J. Coll. Interf. Sci., 2016, 479, 25-33.

5. Cesa, S., Carradori, S., Bellagamba, G., Locatelli, M., Casadei, M.A., Masci, A., Paolicelli,P.:Evaluation of processing effects on anthocyanins content and colour modifications of blueberry (Vacciniumspp.) extracts: comparison between HPLC-DAD and CIELAB analyses” Food Chem., 2017, 232, 114-123

6. Lo Vasco, V.R., Leopizzi M., Di Maio, V., Di Raimo, T., Cesa, S:, Masci, A.: LPS, Oleuropein and Blueberry extracts affect the survival, morphology and Phosphoinositide signalling in stimulated human endothelial cells. Journal of Cell Communication and Signaling, 2017, 11(4) 317-327.

7. Garzoli, S., BožovićM., Baldisserotto, A., Sabatino, M., Cesa, S:,Mai, A., Pepi, F., Vicentini, C.B., Manfredini, S., Ragno R.: Essential Oil Extraction, Chemical Analysis and Anti-CandidaActivity of Foeniculum vulgare Miller– new approaches. Natural Product research 2017, 1-6.

8. Mocan, A., Carradori, S., Locatelli, M., Secci D:, Cesa, S., Mollica, A., Riga, S., Angeli, A., Supuran, C.T., Celia, C., Di Marzio, L.: Bioactive isoflavones from Pueraria lobata root and starch: different extraction techniques and carbonic anhydrase inhibition Food and Chemical Toxicology, 2018, 112, 441-447.

9. Boutaoui, N., Zaiter, L., Benayache, F., Benayache, S., Carradori, S., Cesa, S., ... & Locatelli, M. (2018). Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts. Molecules, 23(2),

10. Masci, A., Carradori, S., Casadei, M.A., Paolicelli, P., Ragno, R., Cesa, S.:Lycium barbarum polysaccharides: Extraction, purification, structural characterisation and evidence about hypoglycaemic and hypolipidaemic effects. A review Food Chemistry,2018, 254, 377-389.

11. Božović, M, Garzoli, S., Baldisserotto, A., Andreotti, E, Pepi, F., Cesa, S., , Vertuani ,S., Manfredini, S., Ragno, R: Melissa officinalisL. subsp. altissima(Sibth. & Sm.) Arcang. essential oil: Chemical composition and preliminary antimicrobial investigation of samples obtained at different harvesting periods and by fractionated extractions. Industrial crops and products, 2018, 317-321.

12. Pacelli, S., Paolicelli, P., Avitabile, M., Varani, G., Di Muzio, L., Cesa, S., ... & Adrover, A. (2018). Design of a tunable nanocomposite double network hydrogel based on gellan gum for drug delivery applications. European Polymer Journal. 2018.

13. Locatelli M., Macchione N., Ferrante C., Chiavaroli A., Recinella L., Carradori S., Zengin, G:,. Cesa S., Leporini L., Leone S., Brunetti L., Menghini L Giustino O. Graminexpollen: phenolic pattern, colorimetric analysis andprotective effects in immortalized prostate cells (PC3) and rat prostatechallenged with LPS Molecules2018.

14. A. P. Sobolev, L. Mannina, N. Proietti, S. Carradori, M. Daglia, A. Maria Giusti, R. Antiochia, D. Capitani "Untargeted NMR-Based Methodology in the Study of Fruit Metabolites, ”Molecules, 20, 4088-4108, 2015

15. L. Mannina, A. P. Sobolev, A. Di Lorenzo, S. Vista, G.C. Tenore, M. Maglia“Chemical Composition of Different Botanical Origin Honeys Produced by Sicilian Black Honeybees (Apis mellifera ssp. sicula)Journal of Agricultural and Food Chemistry, 63, 5864-5874, 2015
16. D. Gorietti, E. Zanni, C. Palleschi, M. Delfini, D. Uccelletti, M. Saliola, C. Puccetti, A. Sobolev, L. Mannina, A. Miccheli, “13C-NMR based profiling unveils different a-Ketoglutarate pools involved into glutamate and lysine synthesis in the milk yeast Kluyveromyces lactis”Biochimica et Biophysica Acta-General Subjects, 1850, 2222–2227, 2015

17. M. P. Donzello, D. Vittori, E. Viola, L. Zeng, Y. Cui, K. . Kadish, L. Mannina, C. Ercolania“Tetra-2,3-pyrazinoporphyrazines with externally appended pyridine rings. 16. A rare class of uncharged water soluble complexes: UV-vis spectral, redox, and photochemical properties”, Journal of Porphyrins and Phthalocyanines, 19, 903–919, 2015

18. G. Adiletta, P. Russo, N. Proietti, D. Capitani, L. Mannina, A. Crescitelli , M. Di Matteo “Characterization of Pears During Drying by Conventional Technique and Portable non Invasive NMR”CHEMICAL ENGINEERING TRANSACTIONS, 44, 151-156, 2015

19. Moreira, G., Fedeli, E., Ziarelli, F., Capitani, D., Mannina, L., Charles, L., Viel, S., Gigmes, D., Lefay, C. “Synthesis of polystyrene-grafted cellulose acetate copolymers via nitroxide-mediated polymerization” Polymer Chemistry, 6, 5244- 5253, 2015

20. S. Carradori, L. Mannina, F. De Cosmi, T. Beccarini, D. Secci, A. P. Sobolev “Optimization of the Microwave-Assisted Extraction of Azadirachta indica (Neem) Leaves Using NMR-based Metabolic Fingerprinting”Current Bioactive Compounds, Vol. 11, pp. 142-145, 2015

21. S. Menta, S. Carradori, D. Secci, C. Faggi, L. Mannina, R. Cirilli, M. Pierini "The anancomeric character of the pharmacophore 1,3,4-thiadiazoline framework in chiral spiro-cyclohexyl derivatives: effects on stereochemistry and spiro-junction lability. Thermodynamic aspects." Journal of Organic Chemistry, 80, pp. 11932−11940, 2015

22. F. Mazzei, G. Favero, P. Bollella, C. Tortolini, L. Mannina, M. Enrique Conti, R. Antiochia“Recent trends in electrochemical nanobiosensors for environmental analysis", International Journal of Environment and Health. 7, 267 – 291, 2015

23. Camin, F., Pavone, A., Bontempo, L., Wehrens, R., Paolini, M., Faberi, A., Marianella, R.M., Capitani, D., Vista, S., Mannina, L. “The use of IRMS, 1H NMR and chemical analysis to characterise Italian and imported Tunisian olive oils” Food Chemistry, 196, pp. 98 – 105, 2016

24. D. Corinti, L. Mannina, B. Chiavarino, Vincent Steinmetz,S.Fornarini, M.E.Crestoni, “IRMPD signature of protonated pantothenic acid, an ubiquitous nutrient” Chemical Physics Letters, 646, 162–167, 2016.

25. L. Mannina, A. P. Sobolev, E. Coppo, A. Di Lorenzo, S. M. Nabavi, A. Marchese, M. Daglia, “Antistaphylococcal activity and metabolite profiling of manuka honey (Lectospermum scoparium L.) after in vitro simulated digestion”.Food & Function 7, 1664-1670, 2016

26. L. Mannina, F. Marini, R. Antiochia, S. Cesa, A. Magrì, D. Capitani, A. P. Sobolev “Tracing the origin of beer samples by NMR and chemometrics: Trappist beers as a case study”, Electrophoresis, 37, 2710-2719, 2016

27. S. Menta, S. Carradori, G. Siani, D. Secci, L. Mannina, A. P. Sobolev, R. Cirilli, M.Pierini, “Elucidation of the mechanisms governing the thermal diastereomerization of bioactive chiral 1,3,4-thiadiazoline spiro-cyclohexyl derivatives towards their anancomeric stereoisomers” RSC Advances, 6, 71262-71272, 2016

28. Laincer, F., Iaccarino, N., Amato, J.,Pagano, B., Pagano, A., Tenore, G., Tamendjari, A., Rovellini, P., Venturini, S., Bellan, G., Ritieni, A., Mannina, L., Novellino, E., Randazzo, A.“Characterization of monovarietal extra virgin olive oils from the province of Béjaïa (Algeria) Food Research International, 89, 1123-1133, 2016

29. R. Nescatelli, S. Carradori, F. Marini, V. Caponigro, R. Bucci, C. De Monte, A. Mollica, L. Mannina, M. Ceruso, C. T. Supuran, D. Secci, “Geographical characterization by MAE-HPLC and NIR methodologies and carbonic anhydrase inhibition of Saffron components”, Food Chemistry, 221, 855–863, 2017

30. A. Vignoli, D. Maria Rodio, A. Bellizzi, A. PSobolev, E. Anzivino, M. Mischitelli, L. Tenori, F. Marini, R. Priori, R. Scrivo, G. Valesini, A. Francia, M. Morreale, M. R. Ciardi, M. Iannetta, C. Campanella, D. Capitani, C.Luchinat, V.Pietropaolo, L. Mannina, “NMR-based metabolomic approach to study urine samples of chronic inflammatory rheumatic disease patients”Anal Bioanal Chem. Vol. 409, 1405–1413, 2017

31. A. P. Sobolev, L. Mannina, M. Costanzo, B. Cisterna, M. Malatesta, C. Zancanaro“Age-related changes in skeletal muscle composition: A pilot nuclear magnetic resonance spectroscopy study in mice”, Experimental Gerontology, 92, 23–27, 2017

32.D. Capitani, A. P. Sobolev, V. Di Tullio, L. Mannina, N. Proietti, “Portable NMR in food analysis”, Chemical and Biological Technologies in Agriculture, 4 (17), 2017

33. A. P Sobolev, S. Circi, D. Capitani, C. Ingallina, L. Mannina “Molecular fingerprinting of food authenticity”, Current Opinion in Food Science, 16, 59–66, 2017

34. Circi, S, Capitani, D. Randazzo, Ingallina, C, Mannina, L., Sobolev, A.P. “Panel test and chemical analyses of commercial olive oils: a comparative study”Chemical and Biological Technologies in Agriculture, 4, 26, 2017,

35. G. N. Manjunatha Reddy, L. Mannina, A. P. Sobolev, S. Caldarelli, “Polyphenols Fingerprinting in Olive Oils through Maximum-Quantum NMR Spectroscopy”, Food Analytical Methods, 11, 2012-2020, 2018

36. A. M. Salzano, A. Sobolev, V. Carbone, M. Petriccione, G. Renzone, D. Capitani, M. Vitale, P. Minasi, M. S. Pasquariello, G. Novi, N. Zambrano, M. Scortichini, L. Mannina, A. Scaloni “A proteometabolomic study of Actinidia deliciosa fruit development”, Journal of Proteomics, 172, 11-24, 2018

37. A. Di Sotto, M. Vecchiato, L. Abete, C. Toniolo, A. M. Giusti, L. Mannina, M. Locatelli, M. Nicoletti, Silvia Di Giacomo, “Capsicum annuum L. var. Cornetto di Pontecorvo PDO: Polyphenolic profile and in vitro biological activities”, Journal of Functional Foods, 40, . 679-691, 2018

38. A. P. Sobolev, L. Mannina, Donatella Capitani, Gabriella Sanzò, Cinzia Ingallina, Bruno Botta, Simonetta Fornarini, M. E. Crestoni, B. Chiavarino, S. Carradori, M. Locatelli, A. M. Giusti, G. Simonetti, G. Vinci, R. Preti, C. Toniolo, M. Reverberi, “A multi-methodological approach in the study of Italian PDO “Cornetto di Pontecorvo” red sweet pepper” Food Chemistry, 255, 120–131, 2018,

39. N. Proietti, G. Adiletta, P.Russo, R. Buonocore, L. Mannina, A. Crescitelli, D. Capitani, “Evolution of physicochemical properties of pear during drying by conventional techniques, portable-NMR, and modelling, Journal of Food Engineering,  2018 doi: 10.1016/j.jfoodeng.2018.02.028

40. C. Fraschetti, L. Guarcini, C. Zazza, L. Mannina, S. Circi, S. Piccirillo, B. Chiavarino A. Filippi “Real time evolution of unprotected protonated galactosamine probed by IRMPD spectroscopy”
Physical Chemistry Chemical Physics, 2018,doiI: 10.1039/C7CP07642H40. G. Testone, A. Sobolev, M. Gonnella,  M. Renna, L. Mannina, D. Capitani, G. Arnesi, T. Biancari.  D.Giannino.“Insights into sucrose pathway of chicory stems by integrative transcriptomic and metabolic analyses”   Phytochemistry,  2019,

41. G. Testone, A. Sobolev, M. Gonnella,  M. Renna, L. Mannina, D. Capitani, G. Arnesi, T. Biancari.  D.Giannino.“Insights into sucrose pathway of chicory stems by integrative transcriptomic and metabolic analyses”   Phytochemistry,  2019,

42. D. Corinti, A. Maccelli , M. E. Crestoni, S. Cesa, D. Quaglio, B. Botta, C. Ingallina, L. Mannina, A. Tintaru, B. Chiavarino, S. Fornarini “IR ion spectroscopy in a combined approach with MS/MS and IM-MS to discriminate epimeric anthocyanin glycosides (cyanidin 3-O-glucoside and -galactoside)”, Int. Journal of Mass Spectrometry, 2019,

43. A. P. Sobolev, F. Thomas, J. Donarski, C. Ingallina, S. Circi, F. Cesare Marincola, D. Capitani, L. Mannina, “Use of NMR applications to tackle future food fraud issues” Trends in Food Science & Technologies, 2019,

44. A. P. Sobolev, A. Ciampa, C. Ingallina, L. Mannina, D. Capitani , I. Ernesti, E. Maggi, R. Businaro, M. Del Ben, P. Engel, A.M. Giusti, L. M. Donini, A. Pinto “Blueberry-based meals for obese patients with metabolic syndrome: A multidisciplinary metabolomic pilot study”Metabolites, 2019, doi:10.3390/metabo9070138

45. A. Di Lorenzo, A. P. Sobolev, S.Fazel Nabavi, A. Sureda, A. Hajizadeh Moghaddam, Sedigheh Khanjani, Jianbo Xiao, S. Shirooie, A.Jorel Tsetegho Sokeng, A. Baldi, L. Mannina, Seyed Mohammad Nabavi, Maria Daglia, “Antidepressive effects of a chemically characterized maqui berry extract (Aristotelia chilensis (molina) stuntz) in a mouse model of Post-stroke depression” Food and Chemical Toxicology, 2019.

46. A. Rotondo, L. Mannina, A. Salvo, “Multiple Assignment Recovered Analysis (MARA) NMR for a Direct Food Labeling: the Case Study of Olive Oils, Food Analytical Methods, 

47. C. Ingallina, A. Cerreto, L.Mannina, S. Circi, S. Vista, D. Capitani, M. Spano, A.P. Sobolev, F. Marini,“Extra-virgin olive oils from nine italian regions: An 1H NMR-chemometriccharacterization” Metabolites, 2019, doi: 10.3390/metabo9040065

48. Salzano, A.M., Renzone, G., Sobolev, A.P., Carbone, V., Petriccione, M., Capitani, D., Vitale, M., Novi, G., Zambrano, N. Pasquariello, M.S., Mannina, L.,  Scaloni, A. “Unveiling kiwifruit metabolite and protein changes in the course of postharvest cold storage”, Frontiers in Plant Science, 2019, doi: 10.3389/fpls.2019.00071

49. A. J. Tsetegho Sokeng,  A. P. Sobolev, A. Di Lorenzo, J. Xiao, L. Mannina, D. Capitani, M. Daglia, “Chemical characterization of fruits and leaves from Adansonia digitata L. (baobab): a multi-methodological approach”, Food Chemistry, 2019doi: 10.1016/j.foodchem.2018.08.030

50. E.Viola, M. P.  Donzello, S. Testani, G. Luccisano,M. L. Astolfi, C. Rizzoli, L. Cong. L. Mannina, C. Ercolani,  K.. M. Kadish, “Tetra-2,3-pyrazinoporphyrazines with Peripherally Appended Pyridine Rings. 19. Pentanuclear Octa(2- pyridyl)tetrapyrazinoporphyrazines Carrying Externally Carboranthiolate Groups: Physicochemical Properties and Potentialities as Anticancer Drugs”, Inorg. Chem., 2019,  doi: 10.1021/acs.inorgchem.8b02269

51. C. Ingallina, D. Capitani, L. Mannina, S. Carradori, M. Locatelli, A. Di Sotto,  S.  Di  Giacomo,  C.  Toniolo,  G.  Pasqua,  A.  Valletta, G.  Simonetti,  A.  Parroni,  M.  Beccaccioli,  G. Vinci,  Mattia  Rapa,  A. Maria Giusti, C. Fraschetti, A. Filippi, A. Maccelli, M. E. Crestoni, S. Fornarini, A. P. Sobolev, “Phytochemical and biological characterization of Italian “sedano bianco diSperlonga” Protected Geographical Indication celery ecotype: a multimethodo-logical approach”, Food Chemistry, 2020, doi 10.1016/j.foodchem.2019.125649

52. C. Ingallina, A. P. Sobolev, S. Circi, M. Spano, C. Fraschetti, A. Filippi 1, A. Di Sotto , S. Di Giacomo, G. Mazzoccanti, F. Gasparrini, D. Quaglio, E. Campiglia, S. Carradori,M. Locatelli, G. Vinci, M. Rapa, S. Ciano, A. M. Giusti, B. Botta, F. Ghirga, D. Capitani, L.Mannina "Cannabis sativa L. Inflorescences from Monoecious, Cultivars Grown in Central Italy: An UntargetedChemical Characterization from Early Flowering to Ripening"Molecules, 2020 doi:10.3390/molecules25081908

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