Nanomedicine_Lab (CHIM/09)

Attività di ricerca

The research activity of the Nanomedicine_Lab is mainly involved in preparation and characterization of innovative drug delivery systems with application in therapeutic, diagnostic and theranostic fields, by different administration routes.
The lab activity focus on the application of different preparation techniques of liposomes, niosomes, nanobubbles and nanoemulsions and their physical-chemical characterization.
In the last years, our research centred on brain delivery, with particular attention to develop nanocarriers able to trespass the Blood Brain Barrier, e.g. amphiphile-coated nanobubbles, or tailored to reach the brain by alternative route, such as nose-to-brain administration (N2B). These strategies provide promising alternatives that are able to ameliorate the treatment of brain disease.
Furthermore, particular attention in formulating nanocarriers and nanovectors is given to natural compounds such as essential oils and to stimuli-responsive molecules (e.g pH-sensitive peptides).

Collaborazioni nazionali e internazionali
  • Prof M. Moghimi School of Medicine, Pharmacy and Health, Durham University, Durham, UK.
  • European Commission Joint Research Centre (JRC), Institute for Health and Consumer Protection, Nanobiosciences Unit Ispra, VA, Italy

  • Dr Carmen Moran Medical Physics in Centre for Cardiovascular Science, The University of Edinburgh, Edinburgh, UK
  • Dr Julie Tzu-Wen Wang Department of Pharmaceutical Technology King's College London, UK
  • Prof Eliana Souto Department of Pharmaceutical Technology Faculty of Pharmacy, University of Coimbra, Portugal
  • Prof Ijeoma Uchegbu, UCL School of Pharmacy, London, UK
  • Prof Yana Reshetnyak, Physics Department, University of Rhode Island, USA
Strumentazione

The main instrumentations available in the Nanomedicine_Lab consist of Zetasizer Nano ZS90 (measurement of the size, electrophoretic mobility of proteins, zeta potential of colloids and nanoparticles), Spectrofluorometer, Differential Scanning Calorimetry and Spray-drying.
In addition an Atomic Force Microscopy (AFM) is available, due to the collaboration with the Research Center on Nanotechnology Applied to Engineering of Sapienza University (CNIS).

Settori ERC
  • LS7_1 - Medical engineering and technology
  • LS7_3 - Pharmacology, pharmacogenomics, drug discovery and design, drug therapy
  • LS7_6 - Gene therapy, cell therapy, regenerative medicine
  • PE5_8 - Intelligent materials – self assembled materials
Finanziamenti

Grants (2015-2018):

2017

  • Antitubercular drug-loaded multi-liposomes vectors as innovative combinatorial therapy for pulmonary tuberculosis
    Phospholipid Research lnstitute, Heidelberg, DE, in collaboration with Prof. F. Bordi (Physic Dept., Sapienza University of Rome)
  •  Investigation of the ability of gas-filled nano-bubbles to deliver hydrophobic and hydrophilic com-pounds to the brain by disruption of the blood brain barrier (BBB) using focused ultrasoundThundar Grant, in collaboration with Dr. Carmel Moran, UK
  •  Delivery of natural bioactive substances with antimicrobial and antitumoral activity by newly formulated nanoemulsions
    Sapienza University of Rome (Ateneo)
  • Novel MmpL3 inhibitors-loaded niosomes to treat tuberculosis via lung delivery
    Interdisciplinary project subjected to external reviewers
    Sapienza University of Rome (Ateneo)
  • Competition RC/EFSA/SCER/2016/01: "Nanotechnology in agri/food/feed products
    EFSA (European Food Safety Agency)

2016

  • Study of a method to characterize the interaction between different polyelectrolites and the biologic mud by zeta potential analyses.
    Research project commissioned by ACEA
  • Coated liposomes and niosomes to enhance pentamidine activity against colorectal cancer
    Sapienza University of Rome (Ateneo)
  • Antimicrobial and antitumoral in vitro activity of natural bioactive compounds delivered by nanoemulsions
    Sapienza University of Rome (Ateneo)

2015

  • A novel pharmacological approach to treat Parkinson's disease: a pre-clinical study on Pentasomes (pentamidine-niosome systems)
    Interdisciplinary project subjected to external reviewers
    Sapienza University of Rome (Ateneo)
  • “Attività antinfiammatoria e antibatterica della lattoferrina somministrata per aerosol nelle infezioni delle vie aeree di modelli murini non FC e FC”
    Fondazione Fibrosi Cistica (FFC#12/2015)
Pubblicazioni

Publications (2015-2018):

1. Viviana De Caro, Anna Lisa Scaturro, Flavia Maria Sutera, Giuseppe Avellone, Gabriella Schiera, Evelina Ferrantelli, Maria Carafa, Valerio Rizzo, Fabio Carletti, Pierangelo Sardo, Libero Italo Giannola N-Valproyl-L-Phenylalanine as New Potential Antiepileptic Drug: Synthesis, Characterization and In Vitro Studies on Stability, Toxicity and Anticonvulsant Efficacy Medicinal Chemistry 2015;11(1):30-40.

2. D. Passeri, F. Rinaldi, C. Ingallina, M. Carafa, M. Rossi, M. L. Terranova, and C. Marianecci Biomedical Applications of Nanodiamonds: An Overview J. Nanosci. Nanotechnol. 15, 972-988 (2015)

3. T. Coviello, A.M. Trotta, C. Marianecci, M. Carafa*, L. Di Marzio, F. Rinaldi, C. Di Meo, F. Alhaique, P. Matricardi Gel-embedded niosomes: preparation, characterization and release studies of a new system for topical drug delivery Colloids and Surfaces B: Biointerfaces 125, 291–299 (Amsterdam, The Netherlands, 2015)

4. Luisa Di Marzio, Marcello Locatelli, Felisa Cilurzo, Donato Cosco, Emanuela Gentile, Daniela Scalise, Maria Carafa, Cinzia Anna Ventura, Mathias Fleury, Christelle Tisserand, Massimo Fresta, Christian Celia, Donatella Paolino Physicochemical Evaluation of Long Term Stability of Prostacyclin Released from Biomedical Device through Turbiscan Lab® Expert Medicinal Chemistry 2015; 11(4), 391 – 399

5. Donato Cosco, Donatella Paolino, Jessica Maiuolo, Luisa Di Marzio, Maria Carafa, Cinzia A. Ventura, Massimo Fresta Ultradeformable liposomes as multidrug carrier of resveratrol and 5-fluorouracil for their topical delivery International Journal of Pharmaceutics, Volume 489, Issues 1–2, 1-10 (2015)

6. C. Dong, S. Corsetti, D. Passeri, M. Rossi, M. Carafa, F. Pantanella, F. Rinaldi, C. Ingallina, A. Sorbo and C. Marianecci Visualization and quantification of magnetic nanoparticles into vesicular systems by combined atomic and magnetic force microscopy
AIP Conf. Proc. 1667, 020011 (2015)

7. I. Venditti, T. F. Hassanein, I. Fratoddi, L. Fontana, C. Battocchio, F. Rinaldi, M. Carafa, C. Marianecci, M. Diociaiuti, E. Agostinelli, C. Cametti, M.V. Russo Bioconjugation of Gold-Polymer Core-Shell Nanoparticles with Bovine Serum Amino Oxidase for Biomedical Applications
Colloids and Surfaces B: Biointerfaces 134 (2015) 314–321

8. Federica Rinaldi , Patrizia N. Hanieh , Carlotta Marianecci, Maria Carafa
DLS Characterization of Non-Ionic Surfactant Vesicles for Potential Nose to Brain Application Nanoscience and Nanometrology 2015; 1(1): 8-14

9. Rosita Primavera, Martina Di Francesco, Antonella De Cola, Vincenzo De Laurenzi, Donatella Paolino, Matteo Ciancaioni, Maria Carafa, Christian Celia, Carmine Di Ilio, Antonio Di Stefano, Massimo Fresta, Marcello Locatelli, Luisa Di Marzio HPLC-FLD and spectrofluorometer apparatus: how to best detect fluorescent probe-loaded niosomes in biological samples
Colloids and Surfaces B: Biointerfaces, Volume 135 (1) 2015, Pages 575-580

10. Carlotta Marianecci, Federica Rinaldi, Patrizia N. Hanieh, Donatella Paolino, Luisa Di Marzio and Maria Carafa Nose to brain delivery: new trends in amphiphile-based “soft” nanocarriers Curr Pharm Des. 2015;21:5225-5232

11. Felisa Cilurzo, Maria Chiara Cristiano, Luisa Di Marzio, Donato Cosco, Maria Carafa, Cinzia Anna Ventura, Massimo Fresta and Donatella Paolino Influence of the Supramolecular Micro-Assembly of Multiple Emulsions on their Biopharmaceutical Features and In vivo Therapeutic Response
Current Drug Targets 2015;16(14), 1612-1622.

12. Carlotta Marianecci; Stefania Petralito; Federica Rinaldi; Patrizia N Hanieh; Maria Carafa
Some recent advances on liposomal and niosomal vesicular carriers Journal of Drug Delivery Science and Technology 32 256-269 (2016)

13. Carlotta Marianecci, Luisa Di Marzio, Elena Del Favero, Laura Cantù, Paola Brocca, Valeria Rondelli, Federica Rinaldi, Luciana Dini, Antonio Serra, Paolo Decuzzi, Christian Celia, Donatella Paolino, Massimo Fresta and Maria Carafa Niosomes as Drug Nanovectors: Multiscale pH-Dependent Structural Response Langmuir, 2016;32(5):1241-9.

14. C. Ingallina, F. Rinaldi, A. Bogni, J. Ponti, D. Passeri, M. Reggente, M. Rossi, A. Kinsner- Ovaskainen, D. Mehn, F. Rossi, B. Botta, M. Carafa, C. Marianecci Niosomal approach to brain delivery: Development, characterization and in vitro toxicological studies International Journal of Pharmaceutics 511(2), 969-982 (2016)

15. Mohan C. Pereira, Monica Pianella, Da Wei, Anna Moshnikova, Carlotta Marianecci, Maria Carafa, Oleg A. Andreev & Yana K. Reshetnyak pHsensitive pHLIP® coated niosomes
Molecular Membrane Biology, 33:3-5, 51-63 (2016) doi: 10.1080/09687688.2017.1342969.

16. Federica Rinaldi, Elena Del Favero, Valeria Rondelli, Stefano Pieretti, Alessia Bogni, Jessica Ponti, François Rossi, Luisa Di Marzio, Donatella Paolino, Carlotta Marianecci, Maria Carafa
pH-sensitive niosomes: effects on cytotoxicity and on inflammation and pain in murine models Journal of Enzyme Inhibition and Medicinal Chemistry IENZ 2017 VOL. 32, NO. 1, 538–546

17. Carlotta Marianecci, Federica Rinaldi, Patrizia Nadia Hanieh, Luisa Di Marzio, Donatella Paolino, Maria Carafa Drug delivery in overcoming the blood–brain barrier: role of nasal mucosal grafting
Drug Design, Development and Therapy 2017:11 325–335

18. Rinaldi, Federica; Hanieh, Patrizia Nadia; Longhi, Catia; Carradori, Simone; Secci, Daniela; Zengin, Gokhan; Ammendolia, Maria Grazia; Mattia, Elena; Del Favero, Elena; Marianecci, Carlotta; Carafa, Maria NEEM OIL NANOEMULSIONS: CHARACTERIZATION AND ANTIOXIDANT ACTIVITY
Journal of Enzyme Inhibition and Medicinal Chemistry (IENZ) 2017 32(1):1265-1273

19. Scazzocchio, F., Mondì, L., Ammendolia, M.G., Rinaldi, F., (...), Fraschetti, C., Longhi, C. Coriander (Coriandrum sativum) Essential Oil: Effect on Multidrug Resistant Uropathogenic Escherichia coli Natural Product Communications 2017 12(4), pp. 623-626

20. Rinaldi F, Hanieh PN, Chan LKN, Angeloni L, Passeri D, Rossi M, Wang JT, Imbriano A, Carafa M, Marianecci C. Chitosan Glutamate-Coated Niosomes: A Proposal for Nose-to-Brain Delivery.
Pharmaceutics. 2018 Mar 22;10(2). pii: E38. doi: 10.3390/pharmaceutics10020038

21. Rosita Primavera, Palumbo Paola, Christian Celia, Felisa Cilurzo, Benedetta Cinque, Elisabetta Carata, Maria Carafa, Donatella Paolino, Maria Grazia Cifone, Luisa Di Marzio
An insight of in vitro transport of PEGylated non-ionic surfactant vesicles (NSVs) across the intestinal polarized enterocyte monolayers European Journal of Pharmaceutics and Biopharmaceutics Volume 127, 2018, 432-442

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